Both players suffered the injuries during training this week and will also miss the Champions League clash with Celtic on Tuesday.
A statement from Milan read: “AC Milan announces Cristian Zaccardo has suffered a sprain in his left ankle.
“The defender’s condition will be evaluated in the next coming days to determine a prognosis.
“Marco Amelia will undergo surgery in his left knee today (Friday) having sustained a meniscus injury.”
The Rossoneri are currently 10th in the Serie A.
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Summer 2016 is set to be a very exciting time for football fans, as this is when the long awaited Euro 2016 tournament kicks off.
Since Guus Hiddink arrived at the helm, Chelsea have improved considerably after a tricky start to the campaign.