The 29-year-old has signed a two-year contract that will keep him at Ajax until the summer of 2013.
Ajax have paid a reported fee of €3.5million for the highly-rated midfielder.
“I want to become champions again,” said Janssen. “I leave Twente with a good feeling, they are a top club. They will be at the top again next season.
“When I arrived there, I had a more controlling role but under Preud’homme I was given more freedom. He [Preud’homme] said from the first day on, ‘You will become the best player in Eredivisie’.”
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The reputation of the Premier League means it is the most popular and one of the most watched leagues in the world.
Five English clubs will compete in the last sixteen of the Champions League for the first time ever as Liverpool, Manchester United, Manchester City, Chelsea and Tottenham face some difficult away matches.