Balotelli strained a muscle in the defeat to Brazil at the weekend and was to miss the Spain semi final on Thursday.
He will now miss the rest of the tournament with his AC Milan team mate Stephan el Shaarawy set to take his place.
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Who of Europe’s top strikers will move to England this summer?
After a dreadful start to their Bundesliga campaign, Borussia Dortmund appear to have weathered the storm after going five matches without defeat.