FIFA general secretary Jerome Valcke confirmed that as Italy are representing Europe as runners up of Euro 2012, they cannot be in the same group as neighbours Spain.
Therefore Uruguay, representing South America as Copa America champions, will be in with Spain, whilst Tahiti, Mexico, Japan and an African nation will all discover who they are paired with on Saturday.
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The reputation of the Premier League means it is the most popular and one of the most watched leagues in the world.
Five English clubs will compete in the last sixteen of the Champions League for the first time ever as Liverpool, Manchester United, Manchester City, Chelsea and Tottenham face some difficult away matches.