The Argentine ace suffered following the Copa del Rey defeat to Real Madrid on Tuesday night.
Messi now faces a race to be fit in time for this weekend’s Clasico in La Liga against Real.
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The reputation of the Premier League means it is the most popular and one of the most watched leagues in the world.
Five English clubs will compete in the last sixteen of the Champions League for the first time ever as Liverpool, Manchester United, Manchester City, Chelsea and Tottenham face some difficult away matches.