Goals from Kevin Prince Boateng and Sulley Muntari give the Italian side a commanding first leg lead.
Barcelona must now find a way to win by at least two clear goals without conceding to progress.
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The reputation of the Premier League means it is the most popular and one of the most watched leagues in the world.
Five English clubs will compete in the last sixteen of the Champions League for the first time ever as Liverpool, Manchester United, Manchester City, Chelsea and Tottenham face some difficult away matches.