The Italian side need to avoid defeat against Borussia Dortmund in order to seal progress to the next round of the Champions League.
Hamsik suffered a foot injury during Napoli’s shock loss to Parma on Saturday.
Hamsik missed training on Sunday and will not be included in Rafa Benitez’s squad for the clash with Borussia Dortmund.
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After a dreadful start to their Bundesliga campaign, Borussia Dortmund appear to have weathered the storm after going five matches without defeat.