Vilanova is missing a first choice defence with Carles Puyol, Eric Abidal and Dani Alves all injured.
“The missing players aren’t an excuse for everything,” Vilanova told a news conference.
“At the end of the day, all the players are Barca players and capable of playing to the highest standard.”
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Five English clubs will compete in the last sixteen of the Champions League for the first time ever as Liverpool, Manchester United, Manchester City, Chelsea and Tottenham face some difficult away matches.