Puyol suffered the injury late on in Barcelona’s victory against Benfica last night.
“We are waiting for more information but he will certainly miss the ‘Clasico’,” Barca coach Tito Vilanova said.
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The reputation of the Premier League means it is the most popular and one of the most watched leagues in the world.
Five English clubs will compete in the last sixteen of the Champions League for the first time ever as Liverpool, Manchester United, Manchester City, Chelsea and Tottenham face some difficult away matches.