Diego Forlan netted the winner near the start of the second half, to put Uruguay in with a fantastic chance of making it through.
Nigeria must now beat Spain in their final game and even then it may not be enough.
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The reputation of the Premier League means it is the most popular and one of the most watched leagues in the world.
Five English clubs will compete in the last sixteen of the Champions League for the first time ever as Liverpool, Manchester United, Manchester City, Chelsea and Tottenham face some difficult away matches.