The England international has been out of action for three months with a hernia surgery and a groin injury.
It was expected that the 32-year-old could make his return from the injury against Spurs after returning to training.
“He is not fit again,” explained Ancelotti. “He is out.
“I can say this with a smile because he trained well but we think it will be a very tough game against Spurs.
“We didn’t have a chance to test him in a game. We have to arrange a game for him, maybe on Thursday, after that he can be available.
“He trained well, he doesn’t have a problem to train, but this is a tough game. So he will play a friendly and be ready for the Man Utd game.”
