The Gunners lost 3-0 in the Carling Cup at Eastlands on Wednesday.
Wenger was unhappy that Hughes stepped into his technical area during the game and the Frenchman insists he would do the same again.
“Tomorrow, by coincidence, I am managing my 500th game at Arsenal and I believe I have shaken hands 497 times,” he said.
“I believe that it is a ceremonial courtesy. But the most important is not the ceremonial but the courtesy of behaviour.
“I don’t deny that I am a bad loser but on this occasion I would have done exactly the same if we had won or lost the game.
“I act in accordance with the principles I think are important on the football pitch and I maintain exactly what I said and did.”
Wenger said, when asked if he had been insulted by the City boss: “I’m used to treating this kind of thing with the needed distance. I do not want to make a fuss.
“There are incidents on the touchline but you never heard me ever say anything about another manager after the game, in a press conference or after during the week.
“I maintain exactly the way I behave and do not regret one second of what I did.”