The Portugal international picked up a shoulder injury in training last week and the injury was worse than first feared.
“After tests we conclude he is unfit to participate in the World Cup,” read a statement from the Portuguese Football Federation.
“The medical file will now be presented to the medical department of Manchester United.
“In light of the report, the Portuguese Football Federation, taking into account the Fifa regulations for the 2010 World Cup, will call-up Ruben Amorim as a replacement.”
